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Frequently Asked Questions About Our Products And Services

Mycoplasmacheck



What mycoplasma species are detected with Mycoplasmacheck?

Mycoplasmacheck can detect all mycoplasma species that contaminate cell cultures. All species, including M. arginini, M. fermentans, M. orale, M. hyorhinis, M. hominis, M. genitalium, M. salivarium, M. synoviae, M. pirum, M. gallisepticum, M. pneumoniae, M. yeatsii, Spiroplasma citri and Acholeplasma laidlawii, are listed in the European Pharmacopoeia 2.6.7. In silico analysis shows that more than 100 additional mollicutes can be detected with Mycoplasmacheck.

How sensitive is the Mycoplasmacheck PCR assay?

The qPCR test can detect a minimum of 10 mycoplasma copies per qPCR reaction.

What starting material is accepted for detection of mycoplasma?

We accept heat-inactivated cell-culture supernatant from any kind of eukaryotic cell line, as long as it is at or below risk group level 2.

How much material should I send for mycoplasma testing?

Only 100-200 µL of heat-inactivated cell-culture supernatant is required.

How should I prepare samples for Mycoplasmacheck?

A detailed protocol of how cells should be grown and how the cell-culture supernatant should be prepared are described in our sample submission guide.

When can I submit a sample for analysis?

You can submit a sample for mycoplasma detection at any time. We do not have pre-set testing dates and we analyse the samples for mycoplasma as soon as we receive them.

How soon do I receive the results?

The results of the test appear in your secure online account within 48 hours after sample receipt.

What do the results of Mycoplasmacheck look like?

The Mycoplasmacheck data report indicates the presence or absence of mycoplasma, as well as the presence or absence of PCR inhibitors. The customer is also given the exclusive opportunity to generate a PDF document containing only user-selected cell line results from any testing period. This customised PDF report can be directly used as a verification certificate for scientific journals and granting agencies.

What happens if my culture is positive for mycoplasma contamination?

In the event of mycoplasma contamination, we recommend simply discarding the infected cell line. If the cell line is of significant value, then mycoplasma elimination procedures could be attempted. Numerous protocols and services for mycoplasma elimination are available and can be found online.

What could lead to PCR inhibition?

Most PCR inhibitors are found in cell cultures with >90% confluence or if suspension cells are present in the supernatant. Please refer to our Sample Preparation guide for instructions on how to best prepare your sample.

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