Not all genes are balanced and easy to work with. Some of them, most probably the important ones, can be really quirky and pretty difficult to handle.
Our complex gene service can handle those unwanted difficult genes. Independent on size, GC content or structure we will synthesise any gene for you.
Submit the sequence of interest in electronic format and we will synthesise your DNA fragment. Every synthetic gene is cloned into a standard vector by default or into a vector of choice, if requested. 100% accuracy of gene synthesis is guaranteed and verified by DNA sequencing.
Eurofins Genomics offer complex gene synthesis services:
- for any size
- for high and low GC contents
- when long repeats are present
- when secondary structures are present
Use our expert consultation on any topic e.g. to find the optimal expression system, to check your sequence in advance, or to design your perfect gene in silico.
You also have the possibility to optimise your sequence using GENEius, our optimisation software.
Scales & possiblities
Plasmid preparation and subcloning adapted just for you.
With our gene synthesis service, we offer a wide range of plasmid preparation scales and different options for your cloning vector.
Choose from several options for your cloning vector:
- Eurofins Genomics standard vector - for free -
- Send us your personal vector
Your gene is normally cloned in a standard vector for free. This vector contains either ampicillin or kanamycin resistance.
- Mini prep scale: 1-10µg plasmid DNA - for free -
- Midi prep scale: 15-50µg plasmid DNA
- Maxi prep scale: 100-500µg plasmid DNA
- Mega prep scale: 500µg-2.5mg plasmid DNA
- Giga prep scale: up to 10 mg plasmid DNA
You receive a free DNA mini prep that is carried out automatically on our robots. If the amount of DNA is insufficient, you can choose one of the scales above.
If you order either the mega or giga prep's, an endotoxin-free preparation procedure is available. For maxi prep scale Animal-free production is available.
Linearisation of your gene
Linearisation of your gene is offered in combination with Mini, Midi and Maxi prep scales.
Completion of the digest will be checked via Agarose gel analysis. Some undigested plasmid might still be present in the final reaction.
For linearised mini- and midiprep plasmids the heat-inactivated digest reaction will be shipped.
Extra purification via spun columns for Mini or Midi preps is possible on request.
If the enzyme cannot be heat-inactivated additional spun column purification is available on request.
For Maxiprep alcohol precipitation is available. Shipment of pellet or linearised plasmid in solution is possible.
The standard service is available with the following enzymes: EcoR1, Nhe1, Not1, SacI, SaII, ScaI, XbaI, XhoI. Other enzymes are possible, but Eurofins will have to check if those enzyme can be made available free of charge. In some cases where Eurofins has to buy the enzyme, the enzyme price will have to be charged additionally.
When you order a "stab culture" you will get the E. coli clone with your respective plasmid in a slant Agar tube that has been incubated at 37°C over-night. The slant Agar tube will be delivered at room temperature.
The clone will not be miniprepped and sequenced anymore (if you want this additional QC, please see glycerlol culture).
The clone can be stored at 4°C for 1-2 weeks. Long time storage is not possible.
If you want to prepare a glycerol culture for long time storage at -80°C, we recommend to streak out the clone on a round Agar plate with the appropriate antibiotic, let it grow over-night at 37°C, start a miniprep culture, let it grow over-night and then prepare a glycerol culture. Eurofins also offers to ship glycerol cultures.
When you order a "glycerol culture" you will receive the E. coli clone with your respective plasmid as a glycerol culture (mix of Mini prep culture and glycerol).
The glycerol culture will be prepared from a miniprep culture of your clone. All Mini prep cultures for the preparation of glycerol culture are miniprepped and one read of DNA sequencing is performed, to ensure identity of your clone.
Glycerol cultures will routinely be delivered at room temperature. If you want a shipment on dry ice, please let us know. We would have to add the costs for the dry ice shipment within the EU. We cannot ship glycerol culture to locations outside Europe.
We would like to remind our customers that plasmid DNA is very stable and can easily be transformed into competent E. coli. If you have this method established in your lab, we recommend to use transformation as an alternative to stab or glycerol culture shipment. Also, Eurofins Genomics can perform this service for you in combination with a plasmid prep (Midi to Giga).