How about a larger size?
The pure preparation of starting materials for further experiments can be really annoying. Why not try a new approach: outsource your plasmid preparation and order a scale that suits your experimental needs.
Plasmid DNA preparation services are required in research laboratories, biotech and pharmaceutical companies for applications such as:
- Production of viral vectors
- Production of antibodies and recombinant proteins
- Production of DNA vaccine
- Cell and gene therapy studies
- Other preclinical studies
Eurofins Genomics offers a variety of plasmid preparation services to cover individual needs:
|Giga Prep I
|Giga Prep II
Together with the different plasmid preparation scales we offer a variety of services:
- Endotoxin-free preparation for Maxi, Mega and Giga Preps
- For maxi prep scale Animal-free production is available
- Maxi, Mega and Giga Preps under sterile conditions
- Bioburden, sterility testing and LAL test of endotoxin level available for Maxi, Mega and Giga Preps
- Restriction enzyme digest as free quality control
- Free plasmid DNA sequencing (1 read)
Our plasmid preparations come with the high quality standards and service options like all other products we offer:
- Convenient online ordering via our Ecommerce System
- Individual service
- Personal support from our experts
- Certified according to ISO 9001 and compliant with GCP
Add-on delivery formats
Linearisation of your gene
Linearisation of your gene is offered in combination with Mini, Midi and Maxi prep scales.
Completion of the digest will be checked via Agarose gel analysis. Some undigested plasmid might still be present in the final reaction.
For linearised mini- and midiprep plasmids the heat-inactivated digest reaction will be shipped.
Extra purification via spun columns for Mini or Midi preps is possible on request.
If the enzyme cannot be heat-inactivated additional spun column purification is available on request.
For Maxiprep alcohol precipitation is available. Shipment of pellet or linearised plasmid in solution is possible.
The standard service is available with the following enzymes: EcoR1, Nhe1, Not1, SacI, SaII, ScaI, XbaI, XhoI. Other enzymes are possible, but Eurofins will have to check if those enzyme can be made available free of charge. In some cases where Eurofins has to buy the enzyme, the enzyme price will have to be charged additionally.
When you order a "stab culture" you will get the E. coli clone with your respective plasmid in a slant Agar tube that has been incubated at 37°C over-night. The slant Agar tube will be delivered at room temperature.
The clone will not be miniprepped and sequenced anymore (if you want this additional QC, please see glycerlol culture).
The clone can be stored at 4°C for 1-2 weeks. Long time storage is not possible.
If you want to prepare a glycerol culture for long time storage at -80°C, we recommend to streak out the clone on a round Agar plate with the appropriate antibiotic, let it grow over-night at 37°C, start a miniprep culture, let it grow over-night and then prepare a glycerol culture. Eurofins also offers to ship glycerol cultures.
When you order a "glycerol culture" you will receive the E. coli clone with your respective plasmid as a glycerol culture (mix of Mini prep culture and glycerol).
The glycerol culture will be prepared from a miniprep culture of your clone. All Mini prep cultures for the preparation of glycerol culture are miniprepped and one read of DNA sequencing is performed, to ensure identity of your clone.
Glycerol cultures will routinely be delivered at room temperature. If you want a shipment on dry ice, please let us know. We would have to add the costs for the dry ice shipment within the EU. We cannot ship glycerol culture to locations outside Europe.
We would like to remind our customers that plasmid DNA is very stable and can easily be transformed into competent E. coli. If you have this method established in your lab, we recommend to use transformation as an alternative to stab or glycerol culture shipment. Also, Eurofins Genomics can perform this service for you in combination with a plasmid prep (Midi to Giga).
Animal-product free preparation
Our plasmid preparations can be ordered endotoxin-free and sterile. As additional service we offer animal-product free preparations in Maxi-scale (100-500 µg).
We have developed an optimised protocol to offer a fully BSE- and TSE-free preparation. Certified "Animal-product free" material (e.g. Glycerol, LB agar, LB media, RNase) is used for the production and specially trained staff performs the preparation in a separate work area.
- Animal-product free Maxi preparation
- 10 days turnaround time
- Endotoxin-free and optionally sterile preparation
- Easy selection of the "Animal-free" option in our Ecom System
- Free restriction enzyme digest and sequencing as quality control
To the best of our knowledge, the prepared DNA is free of animal products.