The one-stop solution for quick and easy oligo analysis
Analyse and calculate your DNA or RNA oligo sequence
The Oligo Analysis Tool gives you the option of checking your oligos before ordering. It also facilitates the set up of experiments by calculating the adequate amounts and dilutions for your oligo solutions.
Determines all physical properties of an oligo such as GC content, melting temperature (Tm), molecular weight (MW) and extinction coefficient (EC). It also displays reverse and complement sequences.
Calculates the required OD (optical density) of the oligo based on your experimental set up to decide which oligo synthesis scale needs to be ordered.
Determines how much volume is needed to dilute a highly concentrated oligo stock solution to a specific lower concentration. This application also supports different units for concentration and volume.
Checks if a self-dimer can be formed using this primer sequence in your PCR. Self-dimers are formed by intermolecular interactions between same sense primers, where the primer is homologous to itself.
Checks if the primer pairs should be used in your PCR. The maximum 3' and 5' annealing scores are calculated as well as the melting temparature difference of the primers. The tool will tell you if the primers should be used in PCR or not.
If you are satisfied with the analysed results, you can order directly the synthesis of the oligo.