LightRun 48 & LightRun 96
Easy Sanger sequencing for high-throughput projects.
Our convenient LightRun plate sequencing service is available for high-throughput Sanger sequencing of 96 samples or 48 samples. You can benefit from cost-effective Sanger sequencing even if you do not have a full microwell plate.
Thanks to prepaid barcodes, the service offers unambiguous sample identification throughout the entire process.
Highlights of LightRun Plate
- Fully automated Sanger sequencing of 96 or 48 samples in microwell plates
- High quality data with up to 1100 Q20 bases
- User-friendly order process
- No online sample assignment necessary
Use our validated SeqPrimer or SeqPrimer NightXpress to ensure perfect sequencing results
Make use from our pre-defined Standard Primers and Standard Primer NightXpress
Product details of the LightRun Plate
Starting material:
Purified plasmid DNA or purified PCR fragments with primer premixed in 96 well PCR plates with V-shaped wells.
Data delivery:
The following files are delivered by default:
- .ab1 files - ABI trace files with all available raw data information
- .scf files -Trace files with base calls and quality values
- .seq files - Text files with clipped & unclipped FASTA sequences
- .phd.1 files - Contains base call and quality information
- .pdf quality report - Trace file with quality scores
- FASTA file with all sequences
Data are stored and available online for 6 months.
Delivery time:
Next business day (Mo - Fri) upon sample receipt
Conditions:
DNA templates must be purified. Sequencing reactions cannot be repeated and all reactions will be charged. Samples are not stored. For samples containing difficult-to-read streches (GC-rich, hairpin structurs...) we recommend our SupremeRun 96 or PlateSeq service.
Sending your samples the right way
Sample requirements:
Take 5 µl purified template DNA with either of following concentration:
- Purified plasmid DNA:
80 - 100 ng/µl
- Purified PCR products:
150-300 bp: 2 ng/µl
300-1000 bp: 12 ng/µl
>1000 bp: 25 ng/µl
Add 5 µl of primer with a concentration of 5 µM (5 pmol/µl).
Please send total sample amount of 10µl per well in a 96 well PCR plate with V-shaped wells.
To ensure the best possible quality of sequence data, the total volume of the sample should not be less than 10 µl.
We recommend measuring the DNA concentration on an agarose gel.
Primer characteristics:
- The melting temperature (TM ) of the primer should be between 52° and 58°C and the length should be between 17 – 19bp. Ideally, the GC content of an 17mer should be 10 G+C; for an 18mer 8 – 9 G+C and for a 19mer 7 – 9 G+C.
- G or C should be at the 3’ end, but not more than 3 Gs or Cs.
- The primer sequence should be a good mix of all 4 nucleotides with no more than 4 identical bases in a row (AAAA or GGGG)