LightRun 48 & LightRun 96
Easy Sanger sequencing for high-throughput projects.
Our convenient LIGHTRUN plate sequencing service is available for high-throughput Sanger sequencing of 96 samples or 48 samples. You can benefit from cost-effective Sanger sequencing even if you do not have a full microwell plate.
Thanks to prepaid barcodes, the service offers unambiguous sample identification throughout the entire process.
Highlights of LIGHTRUN Plate
- Fully automated Sanger sequencing of 96 or 48 samples in microwell plates
- High quality data with up to 1,000 nucleotides in Phred20 quality
- User-friendly order process
- No online sample assignment necessary
Use our validated SeqPrimer or SeqPrimer NightXpress to ensure perfect sequencing results
Make use from our pre-defined Standard Primers and Standard Primer NightXpress
Product details of the LightRun Plate
Starting material:
Purified plasmid DNA or purified PCR fragments with primer premixed in 96 well PCR plates with V-shaped wells.
Data delivery:
- chromatogram (.ab1, ABI file)
- text file (.seq, SEQ file)
- fasta format (.fas, FAS file)
Data are stored and available online for 6 months.
Delivery time:
Next business day (mo - fri) upon sample receipt
Conditions:
Sequencing reactions cannot be repeated and all reactions will be charged for. Your DNA material cannot be stored.
Sending your samples the right way
Sample requirements:
Take 5 µl template DNA with either of following concentration:
- Purified plasmid DNA:
80 - 100 ng/µl
- Purified PCR products:
150-300 bp: 2 ng/µl
300-1000 bp: 12 ng/µl
>1000 bp: 25 ng/µl
Add 5 µl of primer with a concentration of 5 µM (5 pmol/µl).
Please send total sample amount of 10µl per well in a 96 well PCR plate with V-shaped wells.
To ensure the best possible quality of sequence data, the total volume of the sample should not be less than 10 µl.
We recommend measuring the DNA concentration on an agarose gel.
Primer characteristics:
- The melting temperature (TM ) of the primer should be between 52° and 58°C and the length should be between 17 – 19bp. Ideally, the GC content of an 17mer should be 10 G+C; for an 18mer 8 – 9 G+C and for a 19mer 7 – 9 G+C.
- G or C should be at the 3’ end, but not more than 3 Gs or Cs.
- The primer sequence should be a good mix of all 4 nucleotides with no more than 4 identical bases in a row (AAAA or GGGG)