w10.0.27 | c9.0.90.05
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Frequently Asked Questions About Our Products And Services

Does the sequence have an impact on synthesis success?

Eurofins Genomics has many years of experience in oligonucleotide synthesis. Occasionally we can identify oligonucleotides that are difficult to produce, due to their sequences. For example, oligonucleotides with a high percentage of "G" residues, especially stretches of "Gs", can be difficult to synthesise.
Moreover, oligos with four or more "Gs" in a row tend to aggregate and form a "guanine tetraplex" (Poon and MacGregor (1998) Biopolymers 45:427-434). Oligos with self-complementary sequences tend to form aggregates, and this formation can complicate HPSF, HPLC and HYPUR purification.

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