25 years ago site directed mutagenesis was invented and everyone tried to introduce mutations in genes: with more or less success and the time it will take to achieve success is indefinite.
Today we are at the next level of science. Any double stranded DNA sequence can be synthesised de novo: effectively and on schedule.
Receive brand-new synthetic genes in just 6 days - and get a head start on your individual project time table. Improve gene expression and remove bad motifs like secondary structures from your DNA fragments.
Make time your best friend - we will find the correct clone for you.
Read more details about our service portfolio.
Curious about the myths, ranking around gene synthesis? Click here.
Choose your gene
Depending on the length and complexity of a gene or project we offer different categories of synthetic genes.
Standard genes are defined by the following criteria:
- Moderate GC content (35-75%)
- No extensive repeats (>25 bp) or critical hairpin structures
- No extensive homopolymer stretches (>18 bp)
If any of the following descriptions below match your request, our service category complex genes would be most suitable for you:
- Consultation included
- Extreme GC content (very high or low)
- Long repeats or secondary structures are part of the gene
Express genes are availabe from only 4 days and are defined by the following criteria:
- Standard Genes only
- In-house standard vector & miniprep scale
- Order must be placed before 2 p.m.
GeneStrands are a cost-efficient alternative for short genes with no complexities:
- Delivery time: starting from 5 working days
- GeneStrands lengths: 20 - 2000 bp
- Delivered as linear, double-stranded DNA fragments
Choose Gene Evolution libraries for screening projects to improve the properties of your protein of interest. We offer the following services:
- Mutations in single positions
- Mutations in multiple positions
- Mutations within specific domains
- Random substitution
One gene - millions of applications
Synthetic genes: the universal key in molecular biology
Expression improvement in heterologous systems
- Codon usage adaptation
- Promoter and enhancer elements
- cDNA cloning
- qPCR and PCR standards
Optimisation of constructs
- Introduction/removal of good and bad motifs
- Construction of hybrid genes
- Screening for improved properties of proteins
Selected publications about gene synthesis
Gene synthesis: Brieke, C. et al., Rapid access to glycopeptide antibiotic precursor peptides coupled with cytochrome P450-mediated catalysis: towards a biomimetic synthesis of glycopeptide antibiotics. Organic & biomolecular chemistry
Gene synthesis: Takeishi, R. et al., Epimerization at C-3′′ in Butirosin Biosynthesis by an NAD+-Dependent Dehydrogenase BtrE and an NADPH-Dependent Reductase BtrF. ChemBioChem
Gene synthesis: Otte, J. et al., Malonic semialdehyde reductase from the Archaeon Nitrosopumilus maritimus is involved in the autotrophic 3-hydroxypropionate/4-hydroxybutyrate cycle. Aem.ASM
Gene synthesis: Buettner, K. et al., Protein surface charge of trypsinogen changes its activation pattern. BMC biotechnology 14(1)
Gene synthesis: Engeland, C. et al., CTLA-4 and PD-L1 Checkpoint Blockade Enhances Oncolytic Measles Virus Therapy. Molecular Therapy 22(11)
Gene synthesis: Chiwata, R. et al., None of the Rotor Residues of F 1-ATPase Are Essential for Torque Generation. Biophysical journal 106(10)
Gene synthesis: Holmberg, M. et al., A versatile bacterial expression vector designed for single-step cloning of multiple DNA fragments using homologous recombination. Protein expression and purification 98
Gene synthesis: Dietz O. et al., Characterization of the small exported Plasmodium falciparum membrane protein SEMP1. PLOS ONE 9(7): e103272
Gene synthesis: Canali E. et al., A high-performance thioredoxin-based scaffold for peptide immunogen construction: proof-of-concept testing with a human papillomavirus epitope. Nature Scientific Reports 4(4729)
Gene synthesis: Mueller W. et al., Hierarchical organization of multi-site phosphorylation at the CXCR4 C Terminus, PLOS ONE 8(5): e64975
Gene synthesis: Van Noort, V., Bradatsch, B., Arumugam, M., Amlacher, S et al., Consistent mutational paths predict eukaryotic thermostability. BMC Evolutionary Biology, 13(1), 7
Gene synthesis: Carciofi M. et al. Concerted suppression of all starch branching enzyme genes in barely produces amylose-only starch granules. BMC Plant Biology 2012, 12:223
Gene synthesis: Lejonklou M. et al. Accelerated Proliferation and Differential Global Gene Expression in Pancreatic Islets of Five-Week-Old Heterozygous Men1 Mice: Men1 Is a Haploinsufficient Suppressor. Endocrinology April 2012 (Genes purchase/synthesis: kinase complex-associated protein (Ikbkap)
Gene synthesis: Ritari J. et al. Detection of Human Papillomaviruses by Polymerase Chain Reaction and Ligation Reaction on Universal Microarray. PLoS ONE 7(3): e34211. (HPV sequences were synthesised and cloned into plasmid vector)
Gene synthesis: Weber E. et al. Assembly of Designer TAL Effectors by Golden Gate Cloning. PLoS ONE 6(5): e19722
Gene synthesis: Gays F. et al. Comprehensive Analysis of Transcript Start Sites in Ly49 Genes Reveals an Unexpected Relationship with Gene Function and a Lack Of Upstream Promoters. PLoS ONE 6(3): e18475.