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Site Directed Mutagenesis

   Intro & Info

Eurofins Genomics’ site directed mutagenesis (SDM) services are available for all kinds of mutations in plasmids.

By default we will classify your project as "Standard SDM". After submitting your SDM order or quote our team will check your project and if you submitted an order for a complex or multi SDM project (which leads to higher costs) we will contact you immediately.


Settings for our standard SDM are:
  • High copy plasmid (up to 7 kb)
  • Antibiotics: Ampicillin, Kanamycin, Chloramphenicol, Tetracyclin
  • GC content 45 - 75%
  • One target site, max. 150 bp mutations or insertions / deletion
  • No repetitive elements in the mutation region
  • Biosafety level 1

With this order you can introduce several mutations in one plasmid (all of them present in the sequence “mutated construct” given above). All mutations must be in the same plasmid and we will deliver only one end product. Note: If you want to introduce only one mutation at a time and expect us to deliver several end products, please send an email to genes-eu@eurofins.com


Please be aware that the following options are not available for mini and midi prep
  • Endotoxin-free preparation
  • Sterile preparation
  • Animal-free preparation
  • Bioburden test
  • LAL test

Site Directed Mutagenesis Order Form

Target Vector Information open / close
Vector name
Antibiotic Resistance
Vector Size [bp]
bp
Availability
Complete DNA sequence of the wild-type plasmid (sequence of the complete construct)
open / close
Scale
Biosafety Level

Endotoxin-Free Prep

Sterile

Animal-free Prep

Bioburden / Sterility Testing

Endotoxin Testing (LAL)

Insert Information open / close
Position of the mutation
Complete DNA sequence of the mutated construct (the sequence of the final product after mutagenesis)
Upload of data
Base exchanges
Your Project open / close
Remarks:
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