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Cloning Service

   Intro & Info

Eurofins Genomics’ cloning services includes TA cloning, cloning of full length cDNA or PCR products, synthetic genes or subcloning of vector inserts.

By default we will classify your project as "standard cloning" service. After submitting your order or quote our team will check your project and if you submitted an order for a complex cloning service (which leads to higher costs) we will contact you immediately.


Settings for our standard cloning service are:
  • Subcloning of a standard insert (200 – 6,000 bp) or PCR product into a high-copy standard E. coli compatible vector (max. 7000 bp)
  • Subcloning via two different sticky end restriction enzymes
  • Biosafety level 1

Please be aware that the following options are not available for mini and midi prep
  • Endotoxin-free preparation
  • Sterile preparation
  • Animal-free preparation
  • Bioburden test
  • LAL test

Service description
  • Quality control of vector(s)
  • Development of the cloning strategy and subcloning
  • Cloning Verification: We analyse up to 6 clones via restriction digest and/or sequencing of the restriction sites (2 runs)
  • Documentation: By quality assurance report, including project specifications, complete plasmid description, restriction map and DNA sequence information
  • Please note that there can be no guarantee for molecular biology projects
  • In case the subcloning fails even after one repetition we would have to charge a set up fee of 30% per subcloning

Confidentiality

It stands to reason that Eurofins Genomics will perform the services outlined above on a “fee for service” basis. This means that Eurofins Genomics has and will claim no rights whatsoever to all or any of the data that will be generated. Eurofins Genomics will take all necessary measures to ensure that none of the data will be disseminated to third parties.

Cloning Service Order Form

Target Vector Information open / close
Vector name
Antibiotic Resistance
Vector Size [bp]
bp
Copy Number Information
Complete vector sequence
open / close
Scale
Biosafety Level

Endotoxin-Free Prep

Sterile

Animal-free Prep

Bioburden / Sterility Testing

Endotoxin Testing (LAL)

Insert Information open / close
Insert name
DNA sequence of the insert
Upload of data
Donor Vector details
Size of the final contruct
Restriction sites (5')
Restriction sites (3')
Your Project open / close
Remarks:
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