w10.0.26 | c9.0.88.78
PROD | u7.5.14
 

PCR Primer Design

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Instructions here - uc

   PCR Primer Design

Assay Design

Primer Name(s):

1. Target Sequence:


2. Target Sequence:


Design Parameters open / close

1. Target Region (default: complete sequence):

Start:

End:

2. Target Region (default: complete sequence):

Start:

End:

Primer 3' clamp (default: C/G):

Max. Tm difference [°C] between the primers: (default: 2°C)

qPCR Probe Criterias open / close
 

Minimum

Maximum

Probe Length (mer):

Probe GC (%):

Orientation open / close

Please specify the orientation of the qPCR probe on the target sequence:

Sense
Antisense
Primer Criterias open / close
 

Minimum

Maximum

Length (mer):

Primer GC (%):

Tm (°C)

Amplicon Criterias open / close
 

Minimum

Maximum

Size (bp):

GC Content (%):

Tm (°C):

Reaction Conditions open / close

Salt concentration:
(0.1 - 50 mM)

Primer concentration
(0.1 - 50 nM):

Output Parameters open / close

Permit multiple primer binding sites

Yes
No

Sort by Ta of the PCR products

Yes
No


* These fields are mandatory
Design Result
No. Name Sequence Ta Start..End Length GC Tm Order
20 55.0 50.7
Amplicon
xxx
No. Name Sequence Ta Start..End Length GC Tm Order
qPCR Product
xxx
20 55.0 50.7
20 55.0 50.7
No. Name Sequence Ta Start..End Length GC Tm Order
qPCR Product
xxx
20 55.0 50.7
20 55.0 50.7

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