Comprehensive profile of all mutations present in the exome
INVIEW Liquid Biopsy Oncoexome is the first non-invasive service to provide researchers and clinicians an overview of all protein- coding regions in the genome of a suspected cancer patient.
The cancer exome sequencing tool offers the opportunity to discover novel variants, recurrent or frequent somatic mutations in circulating tumour DNA (ctDNA) in cases where limited information on tumour-associated genome instability exists.
- Tumour exome analysis of plasma ctDNA from a non-invasive blood test, where tissue biopsy is not feasible or recommended
- Examination of all mutations in the coding regions of a suspected cancer patient
- Cancer profiling in cases where limited information on tumour-associated mutations is available
- Discovery of structural variants, novel and common genomic alterations
- Comparison of exomes from tissue biopsy and liquid biopsy samples
- Selection of mutations for further analysis
Highlights of INVIEW Liquid Biopsy Oncoexome
- Analysis of novel and known tumour mutations in ctDNA based on next-generation sequencing (NGS)
- Optimised protocols for low-input ctDNA extraction from plasma
- Proprietary workflows for obtaining libraries of high complexity
- Expert data processing with QIAGEN Ingenuity Variant Analysis
Full-service package offered by INVIEW Liquid Biopsy Oncoexome includes the following:
- Cancer exome sequencing of circulating tumour DNA (ctDNA)
- Optimised protocols for library preparation with low duplicate rates
- Exome enrichment of more than 23,000 genes with the Agilent SureSelect V6 platform
- Mutation detection with sensitivity as low as 5% depending on gene loci
- NGS data analysis performed with industry-leading QIAGEN Ingenuity Variant Analysis application
- Entire process carried out according to ISO 17025
Accepted starting material
- 4 mL plasma sample
- 15 ng already isolated cell-free DNA (up to 30 µl / concentration > 0,5 ng/µl)
Illumina 150 bp paired-end sequencing
- Faster identification of relevant variants based on curated biological content from the Ingenuity Knowledge Base
- Prioritisation of most promising variants for further analysis
- Alignment file (bam)
- SNP and InDel tables including annotated variants and effects (vcf, tsv, bed)
- Comprehensive Data Analysis Report (pdf)
The product is available for research use only (RUO).
Our one-stop Diagnostic Solution for tumour exome sequencing of ctDNA isolated from liquid biopsies offers clinicians and researchers stringent quality assurance throughout the entire process. The product includes a range of pre-sequencing services, starting from ctDNA isolation from low-input samples, to library preparation with reduced duplicate rates, as well as superior exon enrichment and uniform coverage with Agilent’s SureSelect latest chemistry.
The next-generation sequencing is carried out under diagnostic conditions and the bioinformatics analysis is performed with the industry’s leading tool, QIAGEN Ingenuity Variant Analysis.