The right answers to frequently asked questions
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What kind of transcriptome analysis service do you offer?
What kind of sample material do you need for transcriptome analysis service?
Please provide us with total RNA.
After an initial quality control step, we isolate polyA+ mRNA from each RNA sample. If requested, we also isolate total RNA from tissue.
How much total RNA do you need for the transcriptome analysis service?
It depends on the type of library to be prepared. Before starting the library preparation, we perform a quality control on each RNA sample. Typical amounts of total RNA for mRNA-Seq libraries are at least 5 µg of high quality total RNA per sample.
In addition Eurofins Genomics does offer you cDNA library protocols for samples with really small amounts of starting material are available, like clinical samples, sorted cells and others. If you need a service partner for such projects just contact us.
What kind of concentration and quality measurement method do you recommend?
To provide you with high quality sequencing data, we need high quality total RNA.
To determine the concentration of your isolated total RNA, we recommend either spectrometric methods like the NanoDrop system or chip-based electrophoretical technologies like the Agilent 2100 Bioanalyzer. Integrity estimations can be done by traditional RNA gel electrophoresis or with the Agilent 2100 Bioanalyzer.
We only start to prepare the library if your RNA sample has passed our QC.
What is the delivery time?
How do you deliver the data?
Depending on the amount of data, you will receive DVDs, an USB stick, or hard disk drives.
In addition to that we offer download of the data from our secure FTP server.
Is it necessary to have a reference sequence for expression profiling?
Yes, we need a reference genome or reference transcriptome sequence to do the mapping of reads and subsequent comparison of expression levels.
What kind of bioinformatic analysis do you offer for transcriptome analysis service?
Typical bioinformatics services after transcriptome sequencing are: