The right answers to frequently asked questions
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How is Inview Transcriptome Isoform Discover different from Inview Transcriptome Explore, Advance and Discover?
Inview Transcriptome Isoform Discover is an RNA-seq standardised service that employs PacBio’s SMRT® technology to profile full-length cDNA transcripts. The product is perfectly suited for thorough investigations of isoform diversity and obtaining a full profile of alternative splicing events in eukaryotic organisms.
Inview Transcriptome Explore, Advance and Discover are standardised products that use Illumina’s platforms to sequence a variety of library types and facilitate studies on gene expression, gene regulation and identification of rare or novel RNA species. The products are applicable to any organism of interest and provide analysis of coding and noncoding RNA.
What starting material will be accepted?
Total RNA extracted from eukaryotic organisms can be used to obtain full-length cDNA sequencing libraries.
How much starting material is required?
For exact information on RNA quantity and concentration, please refer to your quote or contact us directly. In general, we require 3 µg of high- quality RNA with an OD 260/280 1.8-2.2 for optimal results.
The quality and quantity of the provided starting material is essential for the success of sample preparation. The use of too little, degraded or contaminated starting material can result in low yields, cause sample preparation to fail, or compromise the quality and amount of the sequencing results.
How many data packages do I need to reach my experimental goal?
Does Eurofins Genomics offer RNA isolation?
Eurofins Genomics can offer RNA isolation based on optimised protocols as an additional service. Please consult us to discuss possible sample preparation methods for your individual project requirements.
Should I send my starting material on dry ice or would room temperature be sufficient?
RNA must be shipped on dry ice, unless the RNA is precipitated in ethanol. Tissues / cell cultures must be flash- frozen in liquid nitrogen or dry ice and must be mailed on dry ice. Alternatively, fresh material can be stabilised in “RNAlater” (from companies like Ambion, SIGMA or QIAGEN) and be sent at room temperature. Please check in advance if the couriers that you are using accept dry-ice shipments.
What should I do if my sample fails the entry QC?
If the quantity or quality of the starting material does not meet the requirements for further processing, we will contact you directly to discuss how to proceed. If possible, Eurofins Genomics will recommend additional processing steps that could help improve sample quality.
How do I obtain my results and what form will they be in when delivered?
You can download all raw data as well as analysed data in various formats from your secure online account.
Where should I send my samples?
Eurofins Genomics Europe Sequencing GmbH