a) For eukaryotes: rRNA depletion is recommended for (partially) degraded RNA or for when sequencing RNA with no poly-A tail. b) For prokaryotes we recommend rRNA depletion in general, because poly-(A) enrichment of prokaryotic transcripts is not feasible.
You can order rRNA depletion from Eurofins Genomics. Or, alternatively, you can provide us with RNA that has already been rRNA depleted / mRNA enriched as starting material (20 ng per sample (up to 15 µl / concentration >1,4 ng/µl)). Please see Product Specifications for more information.