This depends on many factors, such as tissue-type, sample quality, development status, existing references, etc. Please refer to the table shown under Product Specifications to find your optimum InView™ Transcriptome solution. The estimated read numbers shown there refer to human samples. Alternatively, please contact us to discuss your project. In some cases it might be advisable to set up a trial to define how many reads will be required for your specific aim or organism.
The Inview Transcriptome Explore package gives you an overview of the expressed genes and is ideal for detecting differences between conditions or experiments.
Inview Transcriptome Discover uses a strand-specific RNA library combined with Illumina’s 125 bp paired-end sequencing and is therefore recommended for the detection of differentially expressed genes, rare and novel transcripts and for discovering splice variants. In addition, the information about the transcript’s orientation allows for a more profound determination of gene structure and expression.