Not necessarily. However, if you have a reference, please refer to the following recommendations. A clearly defined Ensembl name (e.g., GRCh37 or Rnor_5.0) for the annotated genomic reference sequence has to be provided prior to project start. Alternatively, the genome sequence can be provided in Fasta (along with the corresponding annotation in Gene transfer format (gtf)) or in GenBank format (including annotation). For projects without a reference genome, de novo transcriptome sequencing based on PacBio RS II technology can be added.