For information on the quantity and concentration of RNA required for specific library preparation methods, please refer to your quote or contact us. In general, we require 150 ng of high-quality RNA (up to 25 µl / optimal concentration 6 - 50 ng/µl (absolute maximum concentration 200 ng/µl)) with an OD 260/280 of 1.8-2.0 and an OD 260/230 of 2.0-2.2 for optimum results. It might be possible to start with less material, but this depends a great deal on the quality of the material and the requested library.
The quality and quantity of the provided starting material are crucial for the success of sample preparation. The use of too little, degraded, contaminated or otherwise damaged starting material can result in a low yield or sample preparation failure and impair the quality and quantity of sequencing results.