The right answers to frequently asked questions
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What kind of samples can be processed with Inview Oncopanel All-In-One?
For Inview Oncopanel, we accept fresh-frozen and FFPE tissue and blood samples.
For liquid biopsy analysis of cell-free DNA (cfDNA) isolated from blood plasma, please refer to our GATCLiquid Oncopanel All-In-One product.
How is the target enrichment performed?
The genes of interests are enriched via a proprietary Eurofins protocols using Agilent SureSelect chemistry. The hybridisation-based strategy eliminates PCR-bias and reduces the number of PCR duplications. The proven target enrichment method has been optimised by Eurofins to develop highly complex libraries from low DNA and to deliver deep and uniform sequence coverage. Following target capture, next-generation sequencing of the library is performed.
What kind of mutations can be detected with Inview Oncopanel All-In-One?
The service fully covers the entire exons of about 600 cancer-relevant genomic regions, including protein-coding genes, select promoter regions and select fusion gene events. In addition to SNPs and InDels, the product can also detect CNVs.
What is the sensitivity of the product?
SNP and InDel detection has a technical sensitivity down to 1%.
What kind of quality controls do you perform?
The quality and quantity of each sample will be determined at sample receipt or after DNA extraction. Further quality controls are performed at various steps of the process.
What is the difference between Inview Oncopanel All-In-One and GATCLiquid Oncopanel All-In-One?
Both tests offer cancer variant detection services based on a validated oncology panel. The interrogated genes and genomic alterations of the panel are the same. Similarly, the techniques used for both products are based on hybridisation for target enrichment and next-generation sequencing.
The two products differ in their starting material. Inview Oncopanel All-In-One can accept conventional source material like fresh-frozen and FFPE tissues, whereas GATCLiquid Oncopanel All-In-One analyses cfDNA extracted from blood plasma samples.
Can I directly compare results between Inview Oncopanel All-In-One and GATCLiquid Oncopanel All-In-One?
Yes, the two services are ideally suited for studies aimed at comparing the performance of traditional biopsies versus liquid biopsies.
What are the limitations of the CNV analysis?
Even when matched samples (tumour tissue and normal tissue or blood from the same patient) are submitted, the paired analysis approach could be subjected to limitations. The measurement variance on this single matched reference sample will be higher than that for a reference consisting of multiple reference samples. This could cause a modest number of (false-positive) CNV calls even in cases where the two paired samples are truly copy number identical.
Will all variants in my sample be detected?
Tumour heterogeneity could make it difficult to correctly identify all relevant copy number variations in a given sample. Even when the tumour itself is homogeneous, excessive contamination of normal cells within a tumour sample could lead to coverage differences that are too small to be detected even with Eurofins Genomics highly sensitive methods.
Can I use Inview Oncopanel All-In-One for diagnostic purposes?
The product is available for research use only (RUO). The service is performed in an ISO17025:2005 –accredited and ISO13485:2016-certified laboratory.
Where do I get my results and in which form are they delivered?
All deliverables can be downloaded via your secure online account.
Where should I send my samples?
Eurofins Genomics Europe Sequencing GmbH