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16S rRNA

The 16S rRNA gene is ubiquitous among all bacteria. Its relatively small size of approximately 1,500 bp and its alternating structure of conserved and hypervariable regions (V1-V9) make the 16S rRNA well suited for identification and phylogenetic analysis of microorganisms. Excellent results are achieved with the V1-V8 primer pair. The option of using the V1-V9 primer pair is also available, but discouraged due to unstable primer attachment to the much smaller V9 region.

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