For sequencing on the PacBio RS II it is crucial that high-quality DNA is used as starting material. The use of too little, degraded, contaminated or otherwise damaged starting material can result in low yield or failure of the sample preparation and impair quality and amount of sequencing results. For optimal results we require at least 10 µg double-stranded, purified, high molecular and RNA-free DNA (concentration approx. 80 ng/µl; OD 260/280 ≥ 1.8; OD 260/230 ≥ 2.0.).