The right answers to frequently asked questions
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Where do I get my results?
All raw data as well as the analysed and assembled data can be downloaded via your secure Ecom online account. Example data of de novo assembly of E. coli DH1 can be viewed in “Files”.
What coverage should I use?
Internal tests showed that a coverage of about 25-30x with corrected PacBio RS II reads is sufficient to produce high-quality genome assemblies. This corresponds to raw data coverage of approx. 100x that should be achieved. Assembly results highly depend on the composition of the analysed genome and may vary between different organisms.
Which starting material should I send and how much of it?
For sequencing on the PacBio RS II it is crucial that high-quality DNA is used as starting material. The use of too little, degraded, contaminated or otherwise damaged starting material can result in low yield or failure of the sample preparation and impair quality and amount of sequencing results. For optimal results we require at least 10 µg double-stranded, purified, high molecular and RNA-free DNA (concentration approx. 80 ng/µl; OD 260/280 ≥ 1.8; OD 260/230 ≥ 2.0.).
Do you guarantee a certain output?
Sequence data are assembled de novo under consideration of all read information, using optimized programs and parameters. The number of contiguous sequences (contigs) that can be unambiguously assembled depends on the complexity (frequency, length and distribution of highly repetitive and duplicate regions) of the sequenced genome and also the quality of the provided starting material. Therefore we cannot guarantee a minimum number of contigs.
What kind of quality controls do you perform?
The quality and quantity of each incoming sample will be determined by appropriate methods. Further quality controls are performed at various steps of the process.
Where should I send my samples?
Eurofins Genomics Europe Sequencing GmbH