The right answers to frequently asked questions
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Which oligo product fits to my application?
As a matter of course the quality of our oligonucleotides should consistently fulfil the performance criteria of your application and arrive in your lab right on time.
For the most common applications like PCR, qPCR, DNA cloning and DNA sequencing by Sanger we offer pre-defined and optimised primer and probes validated in respective applications.
For all applications which are not covered by our Optimised Application Oligos we offer customised DNA & RNA oligos where you can specify your oligo according to your individual requirements.
What does HPSF mean?
HPSF is the abbreviation for Eurofins Genomics proprietary High Purity Salt Free purification method. It is a cartridge purfication technology based on liquid chromatography.
Eight elution steps with different eluents, including acidic on-line cleavage of the dimethoxytrityl (DMT) protecting group guarantees that almost all n-x products, all protecting groups and salts are washed off the raw product.
Multiple HPSF robots in each production facility allow a high throughput purification of thousands of oligos per day.
Which quality controls do you perform?
The final yield of an oligo is determined by measuring the OD (Optical Density) values. One OD260 unit of DNA is the amount of DNA that gives an absorbance reading of 1.0 at a wavelength of 260 nm, for a sample dissolved in 1.0 ml total volume of ddH20 which is read in a 1 cm quartz cuvette. 1 OD260 corresponds to approx. 33 µg/ml of single stranded DNA, depending on the GC content.
To ensure the identity and qualitative purity of each and every synthesised oligonucleotide, we use the latest generation of Sequenom MALDI-TOF (Matrix Assisted Laser Desorption Ionisation - Time of Flight) mass spectrometers. The high grade of automation and the use of proprietary software to analyse the spectra ensures that we deliver oligonucleotides of highest quality.
Capillary Gel Electrophoresis (CGE)
CGE is an effective method for checking the quantitative purity of the synthesised oligonucleotides. It is performed routinely on unmodified oligonucleotides >60 bases which have been purified by RP-HPLC or HYPUR. The electropherograms can be provided along with the synthesis report as quality documentation.