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What does OD mean?

One OD260 (optical density) unit of DNA is the amount of DNA that gives an absorbance reading of 1.0 at a wavelength of 260 nm for a sample dissolved in 1.0 ml total volume of ddH2O, read in a 1 cm quartz cuvette. The OD value is used to determine the concentration of an unknown oligo solution by measuring the absorption at 260 nm.
1 OD260 corresponds to approx. 33 µg/ml of single stranded DNA.

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