For the stock solution we recommend a 10 mM Tris-HCl, pH 8; 1mM
EDTA dilution buffer for most modified oligos.
Oligos labelled with Cyano dyes (Cy3, CY3.5, Cy5 and Cy5.5) are
only stable at pH 7.0. A higher pH would damage the dye
To make the working solution, we recommend to dilute the stock
solution with Tris-buffer. This lowers the EDTA concetration that
may disturb in enzymatical reactions.
In addition, we recommend regularly checking the distilled water
quality which is used for the production of the buffer.
The best way to store the oligos is to aliquot them into several
tubes, and store the aliquots at -20°C. The sample in current use
can be kept in a refrigerator at +4°C for a short time to avoid
continuous freezing and thawing of the solution.
Additionally, we strongly recommend protecting dye labelled
oligonucleotides from light.