5 different sequencing platforms for numerous different applications

Our next generation sequencing service is available on 5 different instruments which allow us to combine our best technologies to offer you the optimum solutions for your applications.

Next generation sequencing is not only deep sequencing but also data analysis and project consulting. We at Eurofins MWG Operon are dedicated to defining projects that perfectly fit all your needs by combining state-of-the-art bioinformatics and different NGS technologies. 

A multitude of well-known and new next generation sequencing (NGS) applications powered by the  Genome Sequencer FLX+, GS Junior, Illumina HiSeq 2000, Illumina MiSeq and PacBio RS are available. Our service is complimented by our extremely flexible service times and our express delivery which is available upon request.

Our NGS services currently offer

• Genome sequencing
• Transcriptome sequencing
• Resequencing & amplicons
• Libraries & bioinformatics

Advantages of our services:

• Fast turnaround times using latest technology standards
• More than 5 years of experience in NGS
• Combined expertise from several partners (e.g. Genomatix, Integrated Genomics)

NGS Favourites

The NGS Favourites are predefined packages which are optimised for specific applications. Our NGS Favourites were developed and continuously extended based on our expertise from several years in business and thousands of successfully finalised projects. Find out more about the NGS Favourites.

Our NGS services can be customised for your individual project needs. Just contact us!

Choose the most suitable instrument for your project

Every next generation sequencing platform has its own advantages. Benefit from full flexibility when choosing Eurofins as your sequencing partner.

Our instrument recommendations for the following applications are available below (click image to enlarge).

Our ratings are based on factors such as data output, read length, turnaround time, data quality and cost efficiency

Specification overview for the different instruments

Depending on your final objective, we will be happy to design the appropriate sequencing experiment for your research that strategically delivers the best possible data quality.

Interested in an individual quote or need additional information? Just contact us!

Selected publications about next generation sequencing

2013

Amplicon analysis: Heinrichs et al. Analysis of Black Fungal Biofilms Occurring at Domestic Water Taps (I): Compositional Analysis Using Tag-Encoded FLX Amplicon Pyrosequencing. Mycopathologia 1-11

Transcriptome analysis: Passos M et al. Analysis of the leaf transcriptome of Musa acuminata during interaction with Mycospharella musicola: gene assembly, annotation and marker development. BMC Genomics 14:78

Genome analysis (Cosmids): Chi X et al. The muraminomicin biosynthetic gene cluster and enzymatic formation of the 2-deoxyaminoribosyl appendage. Med. Chem. Commun., 4, 239-243

2012

Amplicon analysis: Matyasek R et al. Next generation sequencing analysis reveals a relationship between rDNA unit diversity and locus number in Nicotiana diploids. BMC Genomics 13:722

Genome analysis: Stoeckle B et al. Identification of polymorphic microsatellite loci for the endangered Kagu (Rhynochetos jubatus) by high-throughput sequencing. J Ornithol 153:249-253

Transcriptome analysis: Urbarova I et al. Digital Marine Bioprospecting: Mining new neurotoxin drug candidates from the transcriptomes of cold-water sea anemones. Marine Drugs 10(10)

Genome analysis: Witzel K et al. Genome sequence of Enterobacter radicincitans DSM16656T, a plant growth-promoting endophyte. J. Bacteriol. vol. 194

Genome analysis: Zischka M et al. Complete genome sequence of the porcine isolate Enterococcus faecalis D32. J Bacteriol. vol. 194

Amplicon analysis: Jacoby et al. Interindividual variability and co-regulation of DNA methylation differ among blood cell populations. Epigenetics 7:12

Genome analysis: Karczmarczyk M et al. Complete nucleotide sequence of pVQS1 containing a quinolone resistance determinant from Salmonella enterica serovar Virchow associated with foreign travel. J Antimicrob Chemother

Genome analysis: Hauser H, Richter DC et al. Comparative genomic analyses of the Taylorellae. Vet Microbiol. Apr 6

Transcriptome analysis: Mutasa-Göttgens E et al. A new RNASeq-based reference transcriptome for sugar beet and its application in transcriptome-scale analysis of vernalization and gibberellin responses. BMC Genomics 13:99

Genome analysis: Volozhantsev NV et al. Molecular Characterization of Podoviral Bacteriophages Virulent for Clostridium perfringens and Their Comparison with Members of the Picovirinae. PLoS ONE 7(5)

Transcriptome analysis: Hroudova M et al. Diversity, Phylogeny and Expression Patterns of Pou and Six Homeodomain Transcription Factors in Hydrozoan JellyfishCraspedacusta sowerbyi. PLoS ONE 7(4)

Amplicon analysis: Håkansson Å et al. Blueberry Husks and Probiotics Attenuate Colorectal Inflammation and Oncogenesis, and Liver Injuries in Rats Exposed to Cycling DSS-Treatment. PLoS ONE 7(3)

Genome analysis: Morohoshi T et al. Complete genome sequence and characterization of the N-acylhomoserine lactone-degrading gene of the potato leaf-associated Solibacillus silvestris. J Biosci Bioeng. Jan;113(1):20-5

2011

Transcriptome analysis: Windisch H et al. Thermal acclimation in Antartic fish: transcriptomic profiling of metabolic pathways. AJP - Regu Physiol 301, 5

Transcriptome analysis: Kinoshita S et al. Deep Sequencing of ESTs from Nacreous and Prismatic Layer Producing Tissues and a Screen for Novel Shell Formation-Related Genes in the Pearl Oyster. PLoS ONE 6(6)

Genome analysis: Amlacher, S. et al. Insight into structure and assembly of the nuclear pore complex by utilizing the genome of a eukaryotic thermophile. Cell 146, July 2011, p 277-289

Genome analysis: Morohoshi, T. et al. Genome sequence of Microbacterium testaceum StLB037, an N-Acylhomoserine Lactone-Degrading Bacterium Isolated from Potato Leaves. Journal of bacteriology, p. 2072–2073

Transcriptome analysis: Strittmatter, A.W., Richter, D.C., Olbermann, P. Krappmann, S. De novo-Transkriptom- und Expressionsanalyse mit kombinierter NGS. BIOspektrum April 2011, 434-436

Transcriptome analysis: Kuzina V. et al. Barbarea vulgaris linkage map and quantitative trait loci for saponins, glucosinolates, hairiness and resistance to the herbivore Phyllotreta nemorum. Phytochemistry. 2011 Feb 72, 2-3

Genome analysis & gap closing services: Schoen, C.,Weber-Lehmann, J., Strittmatter, A., et al. Whole-genome sequence of the transformableNeisseria meningitidisserogroup A strain WUE2594. Journal of Bacteriology (JB), 27-01-2011

Genome analysis & gap closing services: Rechnitzer, H., Strittmatter, A. et al. Genomic features and insights into the biology of Mycoplasma fermentans. Microbiology, 157, 760–773

2010

Transcriptome analysis: Toulza, E. et al. Gene expression in proliferating cells of the dinoflagellate Alexandrium catenella (Dinophyceae). Applied and Environmental Microbiology, p4521-4529

Amplicon analysis: Medinger, R., Ottenwälder, B. et al. Diversity in a hidden world: potential and limitation of next generation sequencing for surveys of molecular diversity of Eukaryotic microorganisms. Molecular Ecology, 19 Suppl. 1: 32-40

Amplicon analysis: Nolte, V., Ottenwälder, B. et al. Contrasting seasonal niche separation between rare and abundant taxa conceals the extent of protist diversity. Molecular Ecology; 19(14):2908-2915

Fosmid sequencing: Beloqui, A., Strittmatter, AW. et al. Diversity of glycosyl hydrolases from cellulose-depleting communities enriched from casts of two earthworm species. Appl Environ Microbiol. 76(17):5934-46. Epub 2010 Jul 9

Transcriptome analysis: Young, N.D. et al. Elucidating the transcriptome of Fasciola hepatica – A key to fundamental and biotechnological discoveries for a neglected parasite. Biotechnology Advances 28, 222-231

Transcriptome analysis: Elmer KR et al. Rapid sympatric ecological differentiation of crater lake cichlid fishes within historic times. BMC Biol. 2010 May 12; 8:60

Genome analysis: Nowrousian, M. et al. De novo Assembly of a 40 Mb eukaryotic genome from short sequence reads: Sordaria macrospora, a model organism for fungal morphogenesis. PLoS Genetics, April 2010, Volume 6, Issue 4

Transcriptome analysis: Johansen, S.D., Emblem, A., Karlsen, B.O., Okkenhaug, S., Hansen, H., Moum, T., Coucheron, D.H., Seternes, O.M. Approaching marine bioprospecting in hexacorals by RNA deep sequencing. New Biotechnology, March 2010

2009

Genome analysis: De Schutter K., Glinka, S., Weber-Lehmann, J. et al. Genome Sequence of the recombinant protein production hostPichia pastoris. Nature Biotechnology (2009) 27(6), 561-566

Genome analysis: Gordon, P.M. et al. Disease-specific motifs an be identified in circulating nucleic acids from live elk and cattle infected with transmissible spongiform encephalopahties. Nucleic Acids Res (2009) 37(2), 550-556

Genome analysis: Beck, J., Urnovitz, H.B., Riggert, J., Clerici, M., Schütz, E. Profile of circulating DNA in apparently healthy individuals. Clin Chem (2009) 55(4), 730-738

Genome analysis: Steuernagel B. et al. De novo 454 sequencing of barcoded BAC pools for comprehensive gene survey and genome analysis in the complex genome of barley. BMC Genomics 2009, 10:547

2008

Transcriptome analysis: Torres, T.T., Ottenwälder, B. et al. Gene expression profiling by massively parallel sequencing. Genome Research (2008) 18: 172-177

Bold = Eurofins employee

NGS Favourites - straightforward solutions for complex projects

Next generation sequencing service packages predesigned for your application

Many years of experience with next generation sequencing at Eurofins MWG Operon led us to create standard packages for a variety of next generation sequencing applications. Our Favourites are optimised packages for common NGS applications that evolved from the vast array of completed custom projects.

Advantages:

• Highest quality - the NGS Favourites are based on superior in-house techniques which ensure premium data quality
• Economic prices - expertise combined with attractive prices
• Straightforward solutions - save time and effort by choosing a best practices approach that includes all steps to successfully finalise your project
• No hidden costs - all steps from library preparation to bioinformatic analysis are included in our price

NGS Favourites are available for a variety of applications. Find the right Favourite for you application and explore the easy way of sequencing with us as your professional project partner.

1. Genome Sequencing Favourites

   Choose between 2 different types of Favourites based on your project needs. If your project requires high quality de novo assemblies, choose the Favourites using the Roche 454 technology and long paired-end libraries. Our Genome Sequencing Favourites using the Illumina MiSeq are the right choice if shotgun sequencing of small genomes in a short turnaround time is essential for your project.

2. Transcriptome Sequencing Favourites

   Our Transcriptome Sequencing Favourites focus exploring the informative content of the genome at a specific time by sequencing expressed genes. Not only do we analyse your raw data, we will also provide assembled data files or even Excel spreadsheets which can be easily used to interpret your results.

3. Library Service Favourites

   Looking for a competent partner to outsource the library generation of your Roche GS FLX / FLX+ sequencing? Our Library Service Favourites provide quality controlled libraries so you can comfortably sequence the genes of your project needs.

4. Amplicon Sequencing Favourites

   The Amplicon Sequencing Favourites are ideal for detection studies such as metagenome analysis or analysis of somatic mutations. The Roche GS Junior system generates long reads in short turnaround time to facilitate fast variance detection studies.

Didn't find the right Favourite for your project needs? Our experts are standing by to design your tailor-made projects. Just contact us!