Pet breeding: Verification of your pet's pedigree
Confirming the ownership of an individual pet to a pet breed, bloodline or even to a specific sire is of high importance. Hence, Eurofins supports you scientifically to prove the basis of your pet's origin.
Developing and applying DNA markers for marker-assisted identification of breed individuals is getting more efficient with almost daily technology improvements. We support your application in population genetics and in the breeding of:
Do you want to apply established DNA markers in established animals, for example cost-efficient SNP or STR marker genotyping of dogs and cats?
Do you need genetic fingerprints or parentage testing?
Don't hesitate to contact us!
Your question, our answer
The following questions could be arise:
Each genotyping lead to a genetic fingerprint (DNA profil) of the animal that is so individual to be able to identify a single animal out of 6 billion animals. The genetic fingerprint can be perpared from different animal tissues like blood, hair roots, skin, pinfeathers and after slaughtering from meat and meat products. Comparison of several animals or comparison of a single profil with an earlier profile lead to a doubtless identity confirmation. If no reference specimen is available, comparison of the DNA fingerprint with the fingerprint of the parents can be used for parentage confirmation.
In contrast to other marker-based methods (e.g. microchips) the genetic fingerprint is not susceptible for manipulation. Regarding livestock animals, comparison of the DNA profile of meat and meat products with an earlier DNA profile of the living animal enables the detection of the meat origin (see traceability of meat). This unforgeable test supports marketing possibilities.
The genetic fingerprint can only be used for identification and contains no genetic information. Anyhow, the analysis can be combined with a diagnosis of genetic diseases or desired genetic attributes. It is harmless for the animal.
The genetic fingerprint is prepared by using so-called microsatellites. Microsatellites are non-coding regions of the genome where simple DNA sequences, e.g. GA, GAG or CAA, are repeated differently. Hence, the DNA regions have a different length. Each individual shows a unique combination of microsatellite regions of different length that lead to a unique band pattern, like a barcode, in the fragment length analysis. This band pattern is also called DNA profile or genetic fingerprint.
For pets or livestock animals a marker set of at least 10 microsatellites are used to perform individualism successfully.
Animal breeding and animal identification
Genotyping enables a distinct parentage confirmation comparing the DNA profiles of the parents with those of the offspring.
Recovered animals can be identified if the DNA profile is already present or can be prepared from hairs or saliva traces, e.g. on chewing bones.
Additionally, genotyping is the ideal application for seldom or valuable animals like birds or reptiles where microchip methods could be problematic. Here, also sexing is an important application that is possible by genotyping.
Genotyping enables the assignment of meat and meat products to an individual, if the genetic fingerprint was prepared before. If no reference speciment is present, the comparison can be performed facing the sires and/or parents. Hence, a random sample system can be established that enables regular controls.
Another approach is the assignment of a meat or meat product to a specific animal species.
If there is the suspection that protected animal species were found at a retailer or importer, genotyping gives the possibility to detect if the animals are followers (comparison of the DNA profile to the putative sires) or romps.
Sampling is denpendent on the animal species and usage of the samples. The easiest way is to take oral swabs using cotton wool wads that are completely harmless for the animal and especially suitable for small animals.
Often, 1 mL EDTA blood for mammals or 0.1 to 0.2 mL EDTA blood for birds, reptiles, etc. (animals with erythrocytes containing a cell nucleus) are used. Besides, DNA can also be isolated from hair roots and pinfeathers. Using oral swabs, it is important to apply this only for animals that are no longer suckled as maternal cells from the milk in the oral cavity could adulterate the result. Using hair roots, please ensure that the hairs could not originate from other animals that are in contact with the respective animal.
The blood, swab or hair samples can be send unchilled by postal service but should be labeled clearly. A detailed questionnaire can be requested.
Generally, the analysis result is send two weeks after sample reception.
Please request the specific price for your query through our contact form.
For some animals there exist specific contracts with breeding associations.
The results/certificates will be send to the person who submitted the samples. If a breeding association has send the sample the result will be forwarded by the breeding association to the animal owner.
In consultation with the breeding association the results can be entered into a central database, if the animal owner agrees.
If desired, the DNA can be stored for longer time at Eurofins. Please let us know, if this will be required.