In cooperation with our partner AmpTec we can offer you all kinds of in vitro transcripton - RNAs
AmpTec GmbH is a well established service provider for any kind of IVT-RNAs. Together we offer the following product groups:
- single-stranded RNAs of any scale from 100 bp to several thousand bp
- double-stranded RNAs that are synthesised with the ssRNA as a template
- Available in any scale from 100 bp to several thousand bp
- different sets of mRNA for SNF, GPF, ...
- customised mRNA from 1000 bp to several thousand bp
For all products a variety of additional services - like GMP-grade synthesis is available.
The processing of our various RNA products
The following steps describe the processes we take to generate the ssRNA and mRNA products (Research-grade):
- Chemical synthesis of dsDNA fragments and subcloning into a plasmid
- Quality control by sequencing of plasmid inserts
- Amplification of the cloned insert sequence with a universal PCR primer pair
- Purification of the PCR products with spin columns and quality control by capillary electrophoresis
- In vitro transcription of the PCR template
- Removal of the DNA template with DNase treatment
- Purification of the ssRNA with spin columns and quality control by capillary electrophoresis
For dsRNA another in vitro transcription and subsequent quality control will be done using the ssRNA product as a template.