Home sweet home
Need a new home for your gene? Benefit from our wide range of cloning techniques.
Our spectrum of cloning techniques can be used for a multitude of applications:
- Cloning of full length cDNA
Amplification of specific cDNA by RT-PCR and cloning into a vector of your choice
- Cloning of PCR products
Amplification of specific DNA fragments by PCR and cloning into a standard vector or into a vector of your choice
- TA cloning
Cloning of your PCR products into a TA cloning vector
We clone any size of PCR product and your choice of polymerase is unrestricted
- Subcloning of vector inserts
Subcloning of DNA fragments into target vectors, e.g. for in vivo protein expression, or for construction of hybrid genes
- Cloning of synthetic genes
De novo synthesis of DNA fragments or complete genes and cloning into a standard vector or a vector of your choice, e.g. expression vectors
Our experts for cloning will take care of your project and perfom the following steps:
Comprehensive support in the selection of an appropriate cloning strategy
- Experimental performance of the specific DNA amplification
E.g. amplification of complete mRNA sequences by applying optimised RT-PCR technologies
- Gene synthesis
Using our technology, we successfully synthesise DNA fragments and complete genes up to several kb
- Molecular cloning
Cloning of full length cDNAs, synthetic genes, PCR products or vector inserts using standard or more sophisticated molecular cloning techniques
- Quality control
Each vector construct generated at Eurofins Genomics undergoes strict quality control. We perform differential analyses by restriction enzyme digest as well as DNA sequencing to identify correct clones and to verify sequence accuracy.
- Delivery and documentation
The cloned fragments are delivered as E. coli clones or plasmid DNA (mini scale preparation). Higher scales are available on request. The experimental strategy, raw data and final results are summarised in a detailed report.
Strict confidentiality is ensured by every project. The high quality of our service is overseen by a professional quality management. We are certified according to ISO 9001 and compliant with GCP.