Progress or stagnation?
Mutations can either led to a new gene variant or to a defective gene. Gene evolution libraries allow studies to investigate mutations in a defined manner.
Gene evolution libraries are ideally suited for screening projects to improve the properties of your protein of interest.
This technology enables the specific determination of modified nucleotides. The desired mutants are represented in the library.
Available modifications to generate gene evolution libraries:
- Mutations at single positions
- Mutations in multiple positions
- Mutations in specific domains
- Random substitution using degenerated oligonucleotides (wobble oligonucleotides)
Find details about the preparation of gene evolution libraries here.
Processing of your gene evolution library
The following steps describe the generation process of gene evolution libraries:
Consult our experts
- To find the optimal expression system
- To check your sequence in advance
- To design your perfect genes
Using our proprietary software GENEius, the supplied amino acid or DNA sequences are optimised and adapted, e.g. for codon usage to any other organism. GENEius prevents direct or inverted repeats and hairpins and facilitates the even distribution of G‘s and C‘s. Even the incorporation of "good motifs" is easily achieved with the GENEius software. Try our GENEius light and discover what our optimisation software can do for you.
After finalising the project layout we start with the experimental design.We divide the gene in overlapping oligonucleotides for gene synthesis by using GENEius. These oligos will contain wobble positions (e.g. R = G or A; N = A, C, T or G).
After design, the oligonucleotide synthesis phase begins. Our oligonucleotide synthesis facility - with more than 20 years of experience - guarantees highest quality and fast turnaround times.
Our gene synthesis lab is closely linked to the oligonucleotide production. The lab is the perfect combination of robotics and human know-how. Aside from automation, our project managers ensure best practise project layouts and stay in close touch with you if any question arise.
Generation of the library happens during gene synthesis. As we are using wobble bases at certain positions, a pool of DNA pieces is being generated. All pieces are based on the input sequence, but each product contains different mutations at defined locations along its sequence.
Quality that you can count on: the linear synthetic library is being verified by double-strand DNA sequencing.
Your gene evolution library will be delivered linear.
We will provide you with a quality assurance report that contains:
- Detailed project specifications
- Complete plasmid description
- Restriction map
- DNA sequence information
Strict confidentiality is ensured by every project. The high quality of our service is overseen by a professional quality management team. We are certified according to DIN EN ISO 9001:2000.
Scales & possiblities
For our gene evolution libraries we offer you a wide range of plasmid preparation scales.
- Mini prep scale: 1-10µg plasmid DNA - for free -
- Midi prep scale: 15-50µg plasmid DNA
- Maxi prep scale: 100-500µg plasmid DNA
- Mega prep scale: 500µg-2.5mg plasmid DNA
- Giga prep scale: up to 10mg plasmid DNA
You receive a free DNA mini prep that is carried out automatically on our robots. If the amount of DNA is insufficient, you can choose one of the above mentioned scales. For maxi prep scale Animal-free production is available.
If you order either the mega or giga prep's, an endotoxin-free preparation procedure is available.