w6.0.13 | c6.0.57.11
PROD | u6.1.6
 

 

Frequently Asked Questions About Our Products And Services


How is sequence capture on arrays or in solution performed?

  1. The SeqCap EZ oligonucleotide pool is made against target regions of the genome.
  2. A standard shotgun sequencing library is created from genomic DNA.
  3. The sequencing library is hybridised to the SeqCap EZ oligonucleotide pool.
  4. Streptavidin beads are used to pull down the complex of capture oligonucleotides and genomic DNA fragments.
  5. Unbound fragments are removed by washing.
  6. The enriched fragment pool is amplified by PCR.
  7. The success of enrichment is measured by qPCR at control loci.
  8. The end product is a sequencing library enriched for target regions and ready for high throughput sequencing.


NimbleGen Protocol
 

Scroll to top ^^