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Frequently Asked Questions About Our Products And Services



What kind of sample material do you need for sequencing of large constructs like BACs, PACs, fosmids or cosmids?

Please provide us with E. coli glycerol or stab cultures containing the vector constructs.

Is it possible to return the remaining DNA of my BACs, PACs, fosmids or cosmids?

Sure. If requested, we can provide you with the remaining DNA. In this case we will have to charge for handling and shipping of the DNA.

How much sample material do you need for sequencing small genomes like organelle DNA, viruses or phages?

We are able to start with 100-200 ng of DNA. A slight contamination with genomic DNA is acceptable, as long as it is kept <10%. In any case, we recommend sending non-contaminated sample material as this will avoid contaminating sequencing reads.

If possible, we recommend sending between 5-10 µg of sample material. This means we can avoid the amplification step which we have to apply for smaller sample amounts.

What does coverage sequencing mean?

This involves sequencing of a DNA until a certain number of sequence information and therefore sequence coverage is reached.
Example: A 1-fold sequence coverage of a 150 kb BAC in size means 150 thousand base pairs are determined, or a 6-fold coverage means 900 thousand base pairs are determined.

 

How many runs per BACs, PACs, fosmids or cosmids do you need?

It depends on the size of your BACs, PACs, fosmids or cosmids and the required coverage.
Example: A quarter segment of a single run is enough to sequence 24 multiplexed BACs with sizes of up to 150 kb at a 20-fold coverage.

Please refer to your individual quotation.

How many runs per organelle DNA, virus or phage genome do you need?

It depends on the size of your small genome to be sequenced and the required coverage.
Example: 24 genomes of a size of 150 kb can be sequenced in a quarter segment at a 20-fold coverage.

Please refer to your individual quotation.

How do you deliver the data?

Depending on the amount of data, you will receive DVDs, USB sticks or a hard disk drives.

In addition to that we offer download of the data from our secure FTP server.

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