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Frequently Asked Questions About Our Products And Services



Which next generation sequencing services does Eurofins Genomics offer?

Genome sequencing

  • De novo sequencing of fungal genomes
  • De novo sequencing of higher eukaryotic genomes
  • De novo sequencing of BACs, Viruses & Plasmids
  • Re-sequencing of genomes

Transcriptome sequencing

  • De novo transcriptome sequencing
  • Expression profiling

Resequencing & Amplicons

  • Ultra Deep Amplicon Sequencing
  • Resequencing by Sequence Capture
  • Exome Sequencing

Bioinformatics & Special Services

  • Bioinformatic solutions
    like de novo assembly, mapping, transcriptome analysis, amplicon variance analysis ...
  • Library Service
  • Sample Preparation
  • NGS Favourites

How can I order your next generation sequencing service?

As most next generation sequencing projects are very individual, please ask for a personal quote

In close cooperation we will work out your sequencing project.

Where should I send my samples?

Please send your samples together with a copy of your signed project quotation to:

Eurofins Genomics
 NGS-Laboratory
Anzinger Str. 7a
85560 Ebersberg
Germany

Where can I learn more about the various sequencing systems (Illumina, PacBio)?

Illumina:

You can find additional information about this new technique provided by Illumina Inc. at http://www.illumina.com/applications/detail/sequencing/dna_sequencing.ilmn.

PacBio RS:

You can find additional information about this technique provided by Roche Applied Science at http://www.pacificbiosciences.com/products/.

What is the principle of the Illumina sequencing technology?

Both next generation sequencing technologies of Roche (454) and Illumina share a common approach, in, that before sequencing itself, the sequencing library has to be generated via PCR amplification of the DNA templates.

In the case of Illumina HiSeq 2000 or MiSeq, preparation of the sequencing library is done by bridge PCR, while the sequencing is done by a technology referred to as cyclic reversible termination.

In the bridge PCR primers and the DNA template are immobilised to the 2-dimensional surface. The primers are designed to target the adaptors of the DNA fragments so that the fragments bind to primers in their neighbourhood. Within each PCR cycle the fragments build so called bridges and the following denaturation leaves single stranded templates anchored to the surface. The copies remain local and form dense clusters. To sequence the clusters a universal primer is hybridized to the adaptor sequence of the DNA fragments.

FAQ 9.1

Sequencing of clusters generated via bridge PCR is done by a technique called cyclic reversible termination. Thereafter, the polymerase extension is performed with reversible terminators. These are deoxynucleotides carrying a fluorophore and a blocking group. The 4 nucleotides have different fluorophores attached. The polymerase incorporates just 1 labelled nucleotide as the blocking group terminates DNA synthesis. Unincorporated nucleotides are washed away and the array is imaged to determine the identity of the incorporated nucleotide. This is followed by a cleavage step which removes the blocking group and the fluorophore. The resulting 4-colour images are used to decode the sequence.

FAQ 9.2

When using Illumina HiSeq 2500 sequencing, how can you adjust the sequencing data output to my requirements?

Flow cells of the high-output-mode of the Hiseq 2500 instrument are physically subdivided into 8 channels. In 1 channel up to 180 million clusters can be sequenced from one or from both ends.

Given this huge data output, we often recommend pooling of several samples in one channel in order to save sequencing costs. Here we use molecular barcodes (stretches of specific nucleotides) to tag up to 12 unique samples in a single channel. This is appropriate for most applications, like mRNA sequencing or ChIP sequencing.

Can you sequence GC rich DNA or DNA with secondary structures or hairpin structures?

Yes. However, de novo assembly of genomes with high or low GC content remains challenging. In these cases, best assembly results are achieved by sequencing a combination of shotgun and long paired end libraries.

Just contact us for design of the best sequencing strategy

What kind of data do you deliver for Illumina HiSeq and MiSeq projects?

In the case of Illumina HiSeq and MiSeq sequencing we ship FASTQ files of each single read. Intensity values and image files are automatically deleted during the runs and therefore cannot be shipped.

Do you have references for next generation sequencing projects?

Yes, we have reference customers for all our technologies. Please find a selection of all publications here.

If you need a reference for a specific application, please contact genseq-eu@eurofins.com.

How do I provide a reference sequence?

Please send the FASTA or NCBI file or the exact reference to genseq-eu@eurofins.com or to your sales contact person.

Can I get a confidentiality agreement (CDA/NDA)?

Yes, we have templates ready for signature, or we can sign your company/institute agreement, after review.

Confidentiality is guaranteed as part of our quotation and general terms and conditions.

Do I own intellectual property of my results?

Yes, we are a service provider, and as such, we do not claim any intellectual property.

What are NGS Favourites?

Our NGS Favourites are straightforward solutions for complex NGS projects. They are predesigned packages evolved out of the individual project setups from the vast array of custom request.

Learn more about the NGS Favourites here.

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