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PROD | u6.1.6
 

 

Frequently Asked Questions About Our Products And Services


How should Thiol-modified oligos be treated?

We recommend to reduce the thiol before using the oligo in your application:

1. Add 200 µl 10mM TCEP (solve 2,9mg TCEP-HCL in 1ml 1xTE buffer, pH 8.0) to your oligo

2. Shake it for 60 min at room temperature

3. Precipitate the oligo:

  • Add 150 µl 3 M Na-Acetate [24,6 g Na-Acetate + 0,21 g Mg-Acetate in 100 ml dest. H2O]
  • Fill tube with ethanol p.a.
  • Shake gently
  • Incubate 20 min at –20°C

4. Spin for 5 min at 13000 rpm, discard supernatant

5. Dry pellet at room temperature

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