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Frequently Asked Questions About Our Products And Services

How should fluorescent oligonucleotides be stored?

For the stock solution we recommend a 10 mM Tris-HCl, pH 8; 1mM EDTA dilution buffer for most modified oligos.

Oligos labelled with Cyano dyes (Cy3, CY3.5, Cy5 and Cy5.5) are only stable at pH 7.0. A higher pH would damage the dye molecule.

To make the working solution, we recommend to dilute the stock solution with Tris-buffer. This lowers the EDTA concetration that may disturb in enzymatical reactions.

In addition, we recommend regularly checking the distilled water quality which is used for the production of the buffer.

The best way to store the oligos is to aliquot them into several tubes, and store the aliquots at -20°C. The sample in current use can be kept in a refrigerator at +4°C for a short time to avoid continuous freezing and thawing of the solution.

Additionally, we strongly recommend protecting dye labelled oligonucleotides from light.

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