w7.1.04 | c7.1.57.01.04
PROD | u6.1.6
 

 

Frequently Asked Questions About Our Products And Services


How do I calculate the molar amount N of an oligo from the OD value?

For your convenience, please use our online calculation tool MOPS or apply the formula below:

N [nmol] = OD x 100 / 1.54 x nA + 0.75 x nC + 1.17 x nG + 0.92 x nT

n = number of nucleosides of the type X


Example:
Sequence: GAA ATG AGT GCT CAT CAC TAC TTC CGC (27mer)

nA=7; nC=8; nG=5; nT=7
OD=12 (measured)

N [nmol] = 12 x 100 / 1.54 x 7 + 0.75 x 8 + 1.17 x 5 + 0.92 x 7
= 41.3

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