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Frequently Asked Questions About Our Products And Services


How do I calculate the melting temperature TM of an oligo?

The melting temperature TM, characterises the stability of the DNA hybrid formed between an oligonucleotide and its complementary strand.

For your convenience, please use our online calculation tool MOPS or apply the formula below:

Sequences with 15 or less bases 
TM [°C] = 2(nA + nT) + 4(nG + nC)

Sequences with more than 15 bases
TM [°C] = 69.3 + [41(nG + nC) / s - (650 / s)]

n = number of nucleosides of type X 
s = number of all nucleosides per sequence 


Example:
Sequence: GAA ATG AGT GCT CAT CAC TAC TTC CGC (27mer)

nA=7; nC=8; nG=5; nT=7 s= 27

Sequences with 15 or less bases
TM [°C] = 2(7 + 7) + 4(5 + 8) = 80.0

Sequences with more than 15 bases
TM [°C] = 69.3 + [41(5 + 8) / 27] - (650 / 27) = 69.3 + 19.7 - 24.0 = 65.0

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