Looking for the perfect oligo for your cloning applications?
This superior Cloning Oligo is incomparable to any other purified oligo on the market.
Verified performance based on numerous applications such as
- DNA cloning and PCR cloning applications
- Gene synthesis and gene cloning experiments
- Generation of random gene libraries (incl. point mutations)
Results show a considerably increased number of correct clones in comparison to other oligo qualities available today:
- Fewer mutations and base substitutions
- Fewer clones to pick
- Fewer sequencing runs
Modified synthesis process and settings ensure the highest coupling efficiency even for oligos up to 60 mers with or w/o 5'PHOs modification.
Our proprietary "gentle" downstream process
- Ensures the least chemical stress for the oligo to prevent mutations
- Minimises the risk of depurinations (especially in AT-rich sequences)
Easy ordering, MALDI-TOF analysis of every oligo, and online documents complete this product.
The optimised parameters are predefined on the order page. All you need to do is enter the names and sequences of your oligos.
What you can expect from the Cloning Oligo
- Synthesis yield: 15-30 OD
- Oligo length: 15-60 bases
- 5' Phosphate available
- Wobbles (non-defined ratio) possible
- TAT of 2-5 working days
- Delivery: lyophilised or concentration adjusted in tubes
- Quality control by OD measurement and MALDI-TOF MS
All relevant documents are provided in your online account free of charge:
- Oligo synthesis report
- Quality report incl. MALDI-TOF MS spectra
- Delivery note
Additional online features free of charge
Your personal online account offers numerous online features free of charge:
- Selectable label layouts for your oligo tubes
- Complementary sequence calculator with direct ordering
- Various email notification options
- Online status tracking of order process and delivery
- Personal order history with re-ordering option
- Dilution calculator
- Oligo Property Scan tool
Customer feedbacks about our Cloning Oligos
Share your opinion with us by using our feedback sheet.
"We have used the Cloning Oligos with incorporated restriction sites for RT-PCR amplification and subsequent cloning of a 9kb viral RNA. Due to the very limited amounts of starting material we did not perform a comparison with conventional primers. However, using the Cloning Oligos we generated amounts of full-length PCR product sufficient for cloning. Sequencing revealed perfect 5' and 3' ends of the intended product. Subcloning to another vector was also successful, proving that the restriction sites within the oligos were functional. Overall, we are sure that the good quality of the oligos has furthered our research."
Dr. Angelika Ziegler, Julius Kühn-Institut, Bundesforschungsinstitut für Kulturpflanzen, Quedlinburg, Germany
"We are using your Cloning Oligos for standard cloning. Up to now they have been working perfectly. Feel free to quote us on your web site."
Translation of the original German statement; Mr. Andreas Vogel, c-LEcta GmbH, Leipzig, Germany
"The ordered Cloning Oligos were used to generate an expression construct with a flag-tag. For this, the original construct has been cut with HindIII/BamHI and then legated with the two complimentary flag-oligos, which generated the HindIII/BamHI overhangs.
The cloning has worked like a charm. Both clones whose plasmids we have sequenced were correct. In this respect, I am very satisfied with the performance of these oligos….I could envisage to order the Cloning Oligos for similar applications in the future."
Translation of the original German statement; Dr. Peter Lorenz, Universitätsklinik Rostock, Germany
"I am using your Cloning Oligos for direct cloning of short linker sequences, for PCR and subsequent cloning of the PCR products and site-directed mutagenesis.Overall I am very satisfied with the performance of your Cloning Oligos!We have had just one case this week that one cloning oligo has integrated a wrong base, what we have retrieved by sequencing. But this oligo was very long with 58 bases in length - maybe that was the reason. The DNA sequences of two other colonies within the same plate were correct."
Translation of the original German statement; Dr. Kathrin Hölsch, TU München, Garching, Germany
"We have used the Cloning Oligos for several preparations of expression vectors. All these clonings were successful with a good rate of correct clones. Therefore the more expensive Cloning Oligos have paid off. Compared to other factors influencing cloning success such as low competence of bacteria the benefit of higher quality oligonucleotides may not have a major impact, but for difficult clonings they may make the difference."
Dr. Helmut Pospiech, Leibniz Institute for Age Research, Jena, Germany